General description
Primary Rabbit antibodies are widely used in research for various assay techniques. Secondary antibodies that are used to detect rabbit antibodies may lack specificity for rabbit immunoglobulins and in many instances will also recognize other immunoglobulins that appear in the sample being tested. This results rise the need for extensive adsorption of the second antibody. Therefore, using a monoclonal antibody to rabbit IgG which is devoid of a binding capacity to other species can serve as a useful research tool in many applications. Monoclonal Anti-Rabbit IgG (γ-chain specific) recognizes an epitope located on the heavy γ-chain of rabbit IgG. The antibody does not react with rabbit IgA, IgM or light chains. No cross reactivity is observed with IgG from bovine, cat, chicken, dog, goat, horse, human, pig or sheep.
Immunogen
purified rabbit IgG
Application
The antibody is recommended to use in various immunological techniques, including ELISA, Immunoblotting and Immunohistochemistry.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
| biological source | mouse |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | RG-96, monoclonal |
| form | buffered aqueous solution |
| species reactivity | rabbit |
| packaging | antibody small pack of 25 μL |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: suitable,immunohistochemistry: suitable,indirect ELISA: 0.2-0.4 μg/mL using 10 μg/mL rabbit IgG for coating |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
