General description
OPA1 (optic atrophy 1) is a dynamin-related GTPase that plays a role in genome preservation and mitochondrial morphology. Localization of OPA1 is thought to be anchored to the inner membrane of the mitochondria. OPA1 is highly expressed in inner and outer retinal neurons and is important for normal mitochondrial function in those cells. Mutations in OPA1 are the major cause of autosomal dominant optic atrophy. Recent research has found that OPA1 also has the ability to protect neurons from excitotoxic injury and could be a potential clinical target to increase endurance of neurons post brain damage.
~90 and ~100 kDa observed. Six isoforms between 80 kDa and 100 kDa are known to exist (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
Immunogen
Recombinant protein corresponding to mouse OPA1.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OPA1 in COS cells (Prof. Mustapha Oulad-Abdelghani, IGBMC, France).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated OPA1 from mouse brain tissue lysate (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-OPA1 antibody, clone 1OPA-1A8 is validated for use in western blotting, ICC & IP for the detection of OPA1.
Biochem/physiol Actions
This antibody detects six known isoforms of OPA1 (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
Physical form
Mouse monoclonal IgG1κ ascites containing 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OPA1 in 10 µg of human brain tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 10PA-1A8, monoclonal |
| species reactivity | mouse, rat, human |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_598513 |
| UniProt accession no. | P58281 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... OPA1(4976) |
