General description
Homeodomain-only protein (UniProt: Q9BPY8; also known as HOPX, Lung cancer-associated Y protein, Not expressed in choriocarcinoma protein 1, Odd homeobox protein 1) is encoded by the HOPX (also known as HOD, HOP, LAGY, NECC1, OB1) gene (Gene ID: 84525) in human. HOPX is an atypical homeodomain protein that lacks the capacity to bind DNA and instead functions by interacting with transcriptional regulators. It plays crucial roles in organogenesis and its expression is observed in both embryonic and adult stem cells. It is reported to be important for anterior-posterior patterning during organ development and its functional impairment is associated with a variety of diseases, including cancer. It serves as a tumor suppressor that regulates cellular proliferation and metastasis. Down-regulation of HOPX is reported in lung cancer, choriocarcinoma, head and neck squamous cell carcinoma, and oral squamous cell carcinoma. It interacts with serum response factor (SRF) and modulates the expression of SRF-dependent cardiac-specific genes and cardiac development. It prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylases that prevent transcription by SRF. Four isoforms of HOPX have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gokulan, RC., et al. (2022). Cancers (Basel). 14(11); 2764; Ota, C., et al. (2018). Sci. Rep. 8; Article 12983).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
His-tagged, full-length, recombinant human Homeodomain-only protein (HOPX).
Application
Quality Control Testing
Evaluated by Western Blotting in Human Placenta tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HOPX in Human placenta tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected HOPX in Human placenta tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound His-tagged, full-length, recombinant human HOPX with a KD of 5.6 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2L10 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Homeodomain-only protein (HOPX).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2L10, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 8.26 kDa,observed mol wt ~11 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Unknown |
| Protein ID accession no. | NP_001138931 |
| UniProt accession no. | Q9BPY8 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... HOPX(84525) |
