General description
Sphingosine 1-phosphate receptor 4 (S1PR4), otherwise known as EDG6, is present, in varying expression levels, in the adult lymph node, bone marrow, and thymus, as well as fetal liver, lung, and thymus. It is activated by the lysosphingolipid sphingosine 1-phosphate, and is coupled to Gi and G12/13 G proteins and via their various effector molecules, SIP4 regulates cell proliferation, differentiation, motility, and migration.
~42 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human S1PR4/EDG6.
Application
Detect S1PR4/EDG6 using this Anti-S1PR4/EDG6 Antibody, clone 16B9.1 validated for use in Western Blotting & IHC.
Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected S1PR4/EDG6 in human red bone marrow and human tonsil tissues.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot Analysis in rat spleen tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected S1PR4/EDG6 in 10 µg of rat spleen tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 16B9.1, monoclonal |
| species reactivity | rat, human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgMκ |
| NCBI accession no. | NP_003766 |
| UniProt accession no. | O95977 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... S1PR4(8698) |
