General description
85 kDa
Immunogen
His-tagged, full length human IKKα
Application
Anti-IKKα Antibody, clone 14A231 is an antibody against IKKα for use in WB.
Research Category
Signaling
Research Sub Category
Immunological Signaling
Biochem/physiol Actions
Recognizes IKKα.
Physical form
Format: Purified
Protein G purified IgG1 in PBS containing 0.05% sodium azide.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 14A231, monoclonal |
| species reactivity | human, monkey, mouse |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001278 |
| UniProt accession no. | O15111 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CHUK(1147) |
