General description
CD36 is a major 88,000 MW platelet glycoprotein also found on monocytes, macrophages and some endothelial cells. It has multiple diverse roles in physiology. CD36 is a receptor for thrombospondin-1 (TSP-1), functioning as an inhibitor of angiogenesis. It also binds oxidized low density lipoproteins (LDL) and shed photoreceptor outer segments. CD36 is also act an endothelial-CAM acting in platelet adhesion and aggregration. Absence of CD36 protects against the formation of arterial plaques, thus slowing the development of atherosclerosis. CD36 transcription is regulated by the nuclear receptor PPAR-gamma.
~70 kDa observed. The calculated MW of this protein is 53 kDa It runs higher due to glycosolation. Uncharacterized bands may be observed in some lysates.
Immunogen
GST-tagged recombinant protein corresponding to Human CD36.
Application
Research Category
Cell Structure
Research Sub Category
Immunological Signaling
This Anti-CD36 Antibody, clone Long 6 is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of CD36.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CD36 in 10 µg of CD36 transfected MV3 cell lysate.
Western Blotting Analysis: A representative lot detected CD36 in CD36 transfected COS-7 cells (Thorne et al., 2006. DNA AND CELL BIOLOGY. Volume 25, Number 5).
Immunohistochemistry Analysis: A representative lot detected CD36 in GM-CSF elicited macrophages (Thorne et al., 2006. DNA AND CELL BIOLOGY. Volume 25, Number 5).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD36 in GM-CSF elicited macrophages (Thorne et al., 2006. DNA AND CELL BIOLOGY. Volume 25, Number 5).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in THP-1 cells treated with GM-CSF.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected CD36 in 10 µg GM-CSF treated THP-1 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | Long 6, monoclonal |
| species reactivity | human, rat |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000063 |
| UniProt accession no. | P16671 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CD36(948) |
