General description
BioTracker SE Cell Proliferation Kits diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining. Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader.
Spectral Properties
Absorbance: 408nm
Emission: 452nm
Application
Live cell fluorescent imaging
Live cell imaging cell proliferation kit used for fluorescent cell labeling in flow cytometry applications.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye
Physical form
Liquid
Preparation Note
Store BioTracker 405 Blue SE Cell Proliferation Kit -20ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. Ideally the 5 mM DMSO stock solution should be prepared on the day of use. Aliquots may be stored for later use, but activity may be reduced over time. The dyes should only be added to aqueous buffer immediately before staining.
Analysis Note
Spectral Properties
Absorbance: 408nm
Emission: 452nm
Other Notes
1. 10 vials of BioTracker 405 Blue SE Dye (100nmol) (CS224590)
2. 1 vials of Anhydrous DMSO (500µL) (CS224578)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| technique(s) | cell based assay: suitable |
| detection method | fluorometric |
| shipped in | ambient |
| storage temp. | -10 to -25°C |
