General description
Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
Immunogen
Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein
Application
Enzyme Immunoassay (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% Glycerol and 0.09% Sodium Azide.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
Other Notes
Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | FMC75, monoclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human, monkey |
| should not react with | rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Enzyme Immunoassay (0.1 µg/mL) Immunoblotting (0.5 µg/mL) Immunocytochemistry Immunohistochemistry Flow Cytometry ) |
| isotype | IgG1 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
