General description
Calsequestrin (CS also known as CSQ), the major Ca2+ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca2+ binding glycoprotein, which function as an internal Ca2+ store in the lumen of the SR.1-3 In mammals, two forms of the protein exist, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease.
Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca2+ release with the help of triadin and junction proteins.
Immunogen
synthetic peptide corresponding to amino acids 66-84 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in human, rat, and dog calsequestrin-1 and highly conserved in bovine (single amino acid substitution). This sequence is not found in calsequestrin-2.
Application
Anti-Calsequestrin-1 antibody produced in rabbit has been used in immunofluorescence.
Anti-Calsequestrin-1 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction.
Biochem/physiol Actions
Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca2+ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction. Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca2+ release with the help of triadin and junction proteins.†
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| species reactivity | canine, mouse, human, rat |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| concentration | ~1.6 mg/mL |
| technique(s) | western blot: 0.5-1 μg/mL using extracts of rat skeletal muscle S1 fraction |
| UniProt accession no. | P31415 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CASQ1(844) mouse ... Casq1(12372) |
