General description
Anti-HGF (Scatter Factor) (Ab-1), mouse monoclonal, clone 4C12.1, recognizes the ~82 kDa hepatocyte growth factor in macrophages, endothelial & Kupffer cells. It is validated for use in WB & IP.
Purified mouse monoclonal antibody. Recognizes the ~82 kDa HGF protein.
Recognizes the ~82 kDa hepatocyte growth factor protein in macrophages, endothelial, and Kupffer cells.
Immunogen
Human
recombinant, human HGF protein
Application
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Analysis Note
Positive Control
Endothelial cells, Kupffer cells, or macrophages
Other Notes
Antibody should be titrated for optimal results in individual systems.
Toi, M., et al. 1998. Clin. Cancer Res.4, 659.
Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp.212, 133.
Birchmeier, C., et al. 1997. Ciba Found. Symp.212, 16.
Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol.12, 537.
Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp.212, 198.
Rosen, E.M., et al. 1997. Ciba Found. Symp.212, 215.
Vande Woude, G.F., et al. 1997. Ciba Found Symp.212, 119.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4C12.1, monoclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (Immunoblotting (1:200-1:400) Immunoprecipitation (10 µL/mg protein lysate)) |
| isotype | IgG2b |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... HGF(3082) |
