General description
Rabbit polyclonal antibody adsorbed against Aβ1-40 and Aβ1-43, followed by immunoaffinity purification. Recognizes specifically β-amyloid peptide (1-42).
Recognizes specifically β-amyloid peptide (1-42). Does not cross-react with β-amyloid peptide (1-40) or β-amyloid peptide (1-43).
- Antibody Target Gene Symbol: APP
- Target Synonym: AAA, AD1, Adap, AL024401, Amyloid precursor, Amyloidogenic glycoprotein, APP isoform 1, APPI, appican, CTFgamma, CVAP, E030013M08RIK, Nexin II, P3, PN2, PreA4, PROTEASE NEXIN2
- Entrez Gene Name: amyloid beta (A4) precursor protein
- Hu Entrez ID: 351 (Related Antibodies: 171610, PC729,PC152, PC151, PC149, NE1012, 171609, 171608, 171607, 171606, 171605, 171604, 171603, 171598, 171537)
- Mu Entrez ID: 11820
- Rat Entrez ID: 54226
This Anti-β-Amyloid (1-42) Rabbit pAb is validated for use in Dot Blot, ELISA, Radioimmunoassay for the detection of β-Amyloid (1-42).
Immunogen
Human
a synthetic peptide corresponding to amino acids from a C-terminal fragment of human β-amyloid peptide (1-42)
Application
Dot Blot (0.1-0.5 µg/ml)
ELISA (0.1-0.5 µg/ml)
Radioimmunoassay (0.5 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In phosphate buffer, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Savage, M.J., et al. 1998. J. Neurosci.15, 1743.
Borchelt, D.R., et al. 1997. Neuron19, 939.
Citron, M., et al. 1997. Nature Med.3, 67.
Hardy, J. 1997. Trends in Neurosci.20, 154.
Solomon, B., et al. 1997. Proc. Natl. Acad. Sci. USA94, 4109.
Duff, K., et al. 1996. Nature383, 710.
Iwatsubo, T., et al. 1994. Neuron13, 45.
Suzuki, N., et al. 1994. Science264, 133.
This antibody is specific for β-amyloid peptide [1-42] and shows no reactivity to β-amyloid peptide [1-40] or β-amyloid peptide [1-43]. This antibody is recommended for use in the quantitative determination of β-amyloid peptide [1-42]. The sensitivity level for sandwich ELISA is 15-25 pg. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Dot Blot (0.1-0.5 µg/mL) ELISA (0.1-0.5 µg/mL) Radioimmunoassay (0.5 µg/mL)) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... APP(351) |
