General description
Research area: APOPTOSIS
N-terminal His6-tagged recombinant human ROCK-II residues 11-552
Product Source: expressed by baculovirus in Sf21 insect cells
Rho kinase (ROCK) is a type of serine/threonine kinase. There are two isoforms, ROCK1 and ROCK2. In humans ROCK1 and ROCK2 are composed of 33 exons and located on chromosome 18 (18q11.1). The ROCK protein structure is characterized by a catalytic kinase domain and Rho-binding domain (RBD) at N-terminal and pleckstrin-homology (PH) domain and an internal cysteine-rich (CR) domain at C-terminal.
Biochem/physiol Actions
Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC
ROCK (Rho kinase) is activated by interacting with Rho GTPase enzyme. ROCK plays a crucial role in various essential cellular functions like adhesion, contraction, migration, and proliferation. Moreover, it regulates the cytoskeleton and actomyosin contractility during apoptosis. Overexpression of ROCK protein has been observed in various types of cancer such as osteosarcoma, hepatocellular carcinomas, colon and bladder cancers.
Packaging
Also available in 250μg size (14-451M), please inquire for pricing and availability.
Physical form
Ni2+/NTA-agarose
Preparation Note
6 months at -70°C
Analysis Note
routinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| form | liquid |
| shelf life | 6 mo. |
| mol wt | Mw 63.3 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| solubility | soluble |
| NCBI accession no. | NM_005406.2 |
| UniProt accession no. | Q13464 |
| storage temp. | −70°C |
| Gene Information | human ... ROCK1(6093) |
