General description
Mitogen activated protein kinase/ERK kinase (MEK)1 is a hydrophilic non-receptor protein and is a member of dual specificity protein kinase family. It is ubiquitously expressed. MAP2K1 is encoded by the gene mapped to human chromosome 15q22.31.
Application
MEK1, active, GST tagged human has been used to induce kinase reactions in in vitro mitogen activated protein kinase/ERK kinase (MEK)1 kinase assay. It has also been used to activate extracellular-signal-regulated kinase 2 (ERK2) by phosphorylation.
Biochem/physiol Actions
Mitogen activated protein kinase/ERK kinase (MEK)1/2 catalyzes the phosphorylation of tyrosine and then threonine in extracellular-signal-regulated kinase1/2. As an essential component of RAS−RAF−MEK−ERK signaling pathway (MAP kinase signaling cascade), it is involved in the regulation of apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative diseases such as cancer.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
| recombinant | expressed in baculovirus infected Sf9 cells |
| Quality Segment | 200 |
| product line | PRECISIO® Kinase |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 276-374 nmol/min·mg |
| mol wt | ~69 kDa |
| UniProt accession no. | P31938 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... Map2k1(26395) |
