General description
ERG1 encodes the pore forming alpha subunit of the voltage-gated channels that conduct the rapid delayed rectifier potassium current, which leads to termination of the cardiac ventricular action potential. A large number of commercially available therapeutic agents (antiarrhythmics, antihistamines, antibiotics and antidepressants) have been shown to preferentially block ERG1 and cause a disorder of cardiac action potential depolarization that predisposes affected individuals to ventricular arrhythmia and sudden death.
Immunogen
Epitope: C-terminus
Synthetic peptide corresponding to the C-terminus of ERG1 (aa 1145-1159 of HERG1).
Application
Immunoblotting: 5 μg/mL
Immunocytochemistry
Immunohistochemistry on 4% paraformaldehyde fixed tissues.
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Anti-Potassium Channel ERG1 Antibody, C-terminus detects level of Potassium Channel ERG1 & has been published & validated for use in IC, IH, IP & WB.
Biochem/physiol Actions
ERG1 protein. By western blot, antibody recognizes bands at 175 and 205 kDa (glycosylated) or 135 and 165 kDa (deglycosylated).
Physical form
Liquid in PBS with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles
Analysis Note
Control
Positive control tissue: Heart
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse, rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NM_000238.2,NM_172056.1,NM_172057.1 |
| UniProt accession no. | Q12809 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... KCNH2(3757) |
