General description
Apoptosis is an important process by which normal tissue homeostasis and function are maintained. Toso (also known as Fas apoptotic inhibitory molecule 3, Regulator of Fas-induced apoptosis and FAIM3) is a novel regulator of Fas-induced apoptosis in lymphoid cells and when overexpressed in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex.
~60 kDa observed.
Despite its predicted molecular weight of 43 kDa, Toso often migrates at 60 kDa in western blots.
Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to a 13 amino acid peptide near the C-terminus of human Toso.
Application
Anti-Toso Antibody is a highly specific rabbit polyclonal antibody, that targets Fas apoptotic inhibitory molecule 3 & has been tested in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human lung tissue lysate with and without blocking peptide.
Western Blotting Analysis: 1 μg/mL of this antibody detected Toso in human lung tissue lysate with and without blocking peptide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | western blot: suitable |
| UniProt accession no. | O60667 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FAIM3(9214) |
