General description
53-55 kDa (Goyert, 2002)
CD14 is composed of 356 amino acids and is anchored to the cell membrane by linkage to glycosyl phosphatidylinositol. In addition to the 53-55 kDa cell surface form, two soluble forms have been described (Goyert, 2002). CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes (Goyert, 2002). Most tissue macrophages and Langerhans cells also express CD14 (Goyert, 1999 and 2002; Gadd, 1989). Anti-CD14, TUK4, was included in the Fourth, Fifth and Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD14 (Goyert 1995 and 1996).
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Milli-Mark Anti-CD14 -FITC Antibody, clone TUK4 is validated for use in FC for the detection of CD14.
Biochem/physiol Actions
Antibody recognizes Human CD14 antigen.
Physical form
Protein A purified
Purified Mouse monoclonal IgG2a conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Preparation Note
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Analysis Note
Control
PBMCs
Evaluated by flow cytometry using human PBMCs.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | FITC conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | TUK4, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001035110.1 |
| UniProt accession no. | P08571 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CD14(929) |
