General description
Superoxide dismutase [Cu-Zn] (EC 1.15.1.1; UniProt P00441; also known as Cu/Zn superoxide dismutase, Epididymis secretory protein Li 44, hSod1, Indophenoloxidase A, Superoxide dismutase 1) is encoded by the SOD1 (also known as ALS1) gene (Gene ID 6647) in human. Superoxide dismutases (SOD) provide important cellular antioxidant defense against oxidative damage by catalyzing the dismutation (or partitioning) of the superoxide (O2−) radical into either molecular oxygen (O2) or hydrogen peroxide (H2O2). Superoxide dismutases are divided into three families based on the metal cofactor. The Cu/Zn type binds both copper and zinc, the Fe and Mn types bind either iron or manganese, and the Ni type binds nickel. Three forms of SOD are present in mammals, the cytosolic SOD1, the mitochondrial SOD2, and the extracellular SOD3. SOD1 exists in dimeric form, whereas the SOD2 and SOD3 are tetrameric. SOD1 and SOD3 Cu/Zn type, whereas SOD2 is Mn type. Mutations in SOD1 gene have been linked to 20% of amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig disease.
~16 kDa observed
Immunogen
Epitope: C-terminal region
KLH-conjugated linear peptide corresponding to a C-terminal sequence of human Cu/Zn-SOD.
Application
Anti-Cu/Zn-SOD Antibody is an antibody against Cu/Zn-SOD for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cu/Zn-SOD in human cerebral cortex, mouse cerebellum, and mouse kidney tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Cu/Zn-SOD in 10 µg of human brain tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | monkey (based on 100% sequence homology), primate (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_000445 |
| UniProt accession no. | P00441 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SOD1(6647) |
