General description
Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Preparation Note
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
| Quality Segment | 100,200 |
| form | buffered aqueous solution |
| usage | 100 uses (0.1 mg size),20 uses (20 μg size) |
| suitability | suitable for electrophoresis (DNA) |
| storage temp. | −20°C |
