General description
Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.
~59 kDa observed
Immunogen
Epitope: MAP3K2/NBN interaction domain
KLH-conjugated linear peptide corresponding to the MAP3K2/NBN interaction domain of human mSin1.
Application
Detect MAPKAP1/Sin1 using this rabbit polyclonal Anti-mSin1 Antibody, Cat. No. 07-2276-I, validated for use in Western Blotting and Immunoprecipitation.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated mSin1 in 0.5 mg of A431 cell lysate.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Biochem/physiol Actions
Expected to react with spliced isoforms 1, 2, 3, 5, 6, but not 4.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected mSin1 in 10 µg of A431 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, human, mouse |
| species reactivity (predicted by homology) | porcine (based on 100% sequence homology), feline (based on 100% sequence homology), horse (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_001006618 |
| UniProt accession no. | Q9BPZ7 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MAPKAP1(79109) |
