General description
Flotillin-2 (UniProt: Q14254; also known as Epidermal surface antigen (ESA), Membrane component chromosome 17 surface marker 1) is encoded by the FLOT2 (also known as ESA1, M17S1) gene (Gene ID: 2319) in human. Flotillin-2 is a highly conserved monotopic integral membrane protein associated with lipid rafts in cellular membranes. Flotillin-2 consists of an N-terminal stomatin/prohibitin/ flotillin/HflC/K (SPFH) domain and a C-terminal hydrophobic region, which are integral to its functions within membrane microdomains. The protein is widely expressed across various tissues and plays critical roles in cellular processes such as signal transduction, endocytosis, membrane trafficking, and cytoskeletal organization. Flotillin-2 forms hetero-oligomeric complexes with flotillin-1, which together modulate receptor signaling and intracellular trafficking. Flotillin-2 acts as a scaffolding protein within caveolar membranes or caveolae-like structures, facilitating the clustering and signaling of growth factor receptors and other signaling molecules. It participates in diverse processes, including T-cell activation, insulin signaling, and epidermal cell adhesion. Involved in oncogenic signaling, where it contributes to tumor cell motility, invasion, and transformation. In addition, Flotillin-2 has been implicated in axonal growth and neuronal signaling, underscoring its role in neurophysiology. Flotillin-2 localizes predominantly to vesicle-like structures within the cytoplasm and cholesterol-rich lipid rafts in the plasma membrane. Its expression is regulated by various post-translational modifications, including phosphorylation and palmitoylation, which modulate its activity and interaction with signaling pathways. Dysregulation of FLOT2 expression and function has been linked to pathological conditions such as inflammatory bowel diseases, cancer progression, and metabolic disorders. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human Flotillin-2.
Application
Quality Control Testing
Evaluated by Western Blotting in HDLM-2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flotillin-2 in HDLM-2 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Flotillin-2 in mouse brain and a 1:1,000 dilution in rat testis tissue lysate.
Affinity Binding Assay: A representative lot of this antibody bound with Flotillin-2 peptide with at least ten million-fold (10,000,000X) higher affinity than with non-specific control peptide.
Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Flotillin-2 in HDLM-2 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Flotillin-2 in HDLM-2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1L10 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Flotillin-2. It targets an epitope within 25 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1L10, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 47.06 kDa,observed mol wt ~50/55 kDa |
| purified by | using Protein A |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | zebrafish, bovine |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_004466 |
| UniProt accession no. | Q14254 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... FLOT2(2319) |
