General description
Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olg-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.
~32 kDa observed.
Immunogen
Recombinant mouse Olig-2.
Application
Anti-Olig-2 Antibody Alexa Fluor™ 555 Conjugate is an antibody against Olig-2 for use in Immunofluorescence.
Research Category
Neuroscience
Research Sub Category
Transcription Factors
The unconjugated antibody (Cat. No. AB9610) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications.
Biochem/physiol Actions
The unconjugated antibody (Cat. No. AB9610) recognizes the ~32 kDa Olig-2 protein by Western blotting.
Physical form
Ammonium Sulfate Precipitation
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunofluorescence in mouse brain tissue.
Immunofluorescence Analysis: An 1:150 dilution of this antibody detected Olig-2 in mouse brain tissue.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 555 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat, mouse, human |
| technique(s) | immunofluorescence: suitable |
| NCBI accession no. | NM_016967.2 |
| UniProt accession no. | Q9EQW6 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... OLIG2(10215) |
