General description
Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
~ 38kDa
Immunogen
Epitope: C-terminus
The immunogen used to the purified peptide conjugated to KLH corresponding to the C-terminus of PP1 beta catalytic subunit (amino acids 318-327).
Application
Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-15μg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
This Anti-PP1β Antibody is validated for use in WB, IP, IH(P) for the detection of PP1β.
Biochem/physiol Actions
Recognizes Protein Phosphatase 1β. No cross reactivity with other recombinant PP1 isoforms.
Physical form
Format: Purified
Purified
Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa Cell Lysate
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB4083
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | bovine, mouse, human, rat |
| manufacturer/tradename | Upstate® |
| technique(s) | immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_206877 |
| UniProt accession no. | P62140 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP1CB(5500) |
