General description
Pleiotrophin (PTN) also called Neurite Growth-promoting Factor 1 (NEGF1) or HARP (Heparin affin regulatory peptide) is a new member of the heparin-binding neurotrophic factor family. PTN plays an important role in development processess and promotion of neurite extension. During embryonic development, pleiotrophin is expressed in the central and peripheral nervous system, lung, kidney, gut and bone. Pleiotrophin is also expressed by several tumor cells and is thought to be involved in tumor angiogenesis. PTN initiates an angiogenic switch in different cancer models and acts as an angiogenic factor. PTN does this through an interaction with endothelial cells to initiate proliferation, migration, and tube formation.
~20 kDa observed
Immunogen
Recombinant protein corresponding to chicken Pleiotrophin.
Application
Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Pleiotrophin in papillary squamous cell carcinoma tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Developmental Neuroscience
This Anti-Pleiotrophin Antibody, clone 3B10 is validated for use in IH, WB for the detection of Pleiotrophin.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Human placenta tissue lysate
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Pleiotrophin in 10 µg of human placenta tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABD179
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3B10, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | XP_416358 |
| UniProt accession no. | P32760 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PTN(5764) |
