SMC® Human IFN-γ High Sensitivity Immunoassay Kit，1EACH，03-0181-00，密理博

General description

Interferon-gamma (IFN-γ) is a key cytokine produced primarily by T cells and natural killer (NK) cells that plays a vital role in the immune response. It is essential for enhancing the immune system′s ability to fight infections, particularly by activating macrophages and promoting the differentiation of T cells into Th1 cells. IFN-γ also has antiviral properties and is involved in the regulation of various immune and inflammatory processes, making it a critical factor in both innate and adaptive immunity.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

• Ultra-Sensitive Detection of IFN-γ: Enables precise quantification of low-level IFN-γ, critical for studying early immune activation, cytokine signaling, and therapeutic response.
• Application in Autoimmune and Inflammatory Disease Research: Supports mechanistic studies of inflammation and immune regulation, including diseases like multiple sclerosis, rheumatoid arthritis, and Castleman disease.
• High Precision and Recovery: Intra-assay CV <15%, inter-assay CV <20%, and recovery rates of 98% in plasma and 104% in serum. Ensures reliable and reproducible data, critical for longitudinal studies, and biomarker validation.
• Specificity for Human IFN-γ: Provides accurate quantification of IFN-γ, supporting mechanistic studies of Th1 responses, macrophage activation, and MHC expression.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 30 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 20-25,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 96% Plasma: 100%
• Recovery: Serum: 104% Plasma: 98%

Other Notes

Although IFN-γ was detectable in non-human primate, mouse, rat, canine and feline samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.