General description
CD70 is an interesting membrane bound cellular factor that acts as a cytokine which binds the receptor CD27. Binding induces the proliferation and co-stimulation of T cells and enhances the generation of cytolytic T-cells. Thus, CD70 plays important roles in T cell adaptive T cell response as well as in innate immunity and is required for the proper activation of the anti-viral adaptive response. CD70 also serves as a regulatory role in early cytokine responses to pathogens through the maintenance of Treg cell survival and function. Recent research also indicates that CD70 expression could be important in autoimmune disease regulation and in treatment of autoimmune diseases such as rheumatoid arthritis. CD70 is expressed on T cells, splenocytes, and thymocytes and it belongs to the TNF family of cellular signaling proteins.
~27 kDa observed
Immunogen
Epitope: Extracellular domain
Recombinant protein corresponding to the extracellular domain of mouse CD70.
Application
Anti-CD70 Antibody, clone TAN 1-7 | MABF112 is an antibody against CD70 Antibody for use in Western Blotting, Flow Cytometry.
Flow Cytometry Analysis: 2 µg of this antibody detected CD70 in mouse bone marrow dendritic cells treated with 2000U/mL GM-CSF and 0.1 ug/mL of LPS.
Functional Blocking Analysis: A representative lot detected CD70 in splenic CD8a+ DCs (Arora, P., et al. (2014). Immunity. 40:105-116).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse spleen tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CD70 in 10 µg of mouse spleen tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | TAN 1-7, monoclonal |
| species reactivity | mouse |
| technique(s) | flow cytometry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_035747 |
| UniProt accession no. | O55237 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cd70(21948) |
