General description
Stromal interaction molecule 2 (STIM2) plays an important role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. It inhibits STIM1-mediated store-operated Ca++ entry; however, STIM2 can form complexes with STIM1. This suggests that STIM1 and STIM2 may play a coordinated role in controlling Ca++ entry. STIM2 is expressed in all tissues and tumor cell lines examined.
~98 kDa observed
Immunogen
Epitope: Intermediate domain
KLH-conjugated linear peptide corresponding to the intermediate domain of human STIM2.
Application
Detect Stromal interaction molecule 2 using this rabbit polyclonal antibody, Anti-STIM2 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected STIM2 in human spleen tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected STIM2 in human spleen cells.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Biochem/physiol Actions
This antibody recognizes the intermediate domain of STIM2.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A-20 cell lysate
Evaluated by Western Blotting in A-20 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected STIM2 in 15 µg of A-20 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| UniProt accession no. | Q9P246 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... STIM2(57620) |
