General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa observed
Immunogen
A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
Epitope: Ser10
Application
Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 488, is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p,
is labeled with Alexa Fluor
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Histones
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
Broad species cross-reactivity is expected.
Histone H3 phosphorylated at Ser10
Physical form
Protein G Purified
Purified mouse IgG1k conjugated to Alexa Fluor™ 488 in PBS with 15mg/mL BSA and less than 0.09% sodium azide.
Preparation Note
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
Analysis Note
Control
Jurkat Cells
Evaluated by Flow Cytometry with Jurkat cells.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3H10, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NM_003493 |
| UniProt accession no. | Q16695 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer10) |
| Gene Information | human ... HIST1H3F(8968) |
