规格
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|
Assay:
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β-Gal (lacZ) Assay |
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Excitation/Emission:
|
492⁄517 |
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Product Line:
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DetectaGene™ |
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Shipping Condition:
|
Room Temperature |
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Substrate:
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CMFDG |
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Substrate Properties:
|
Chemical Substrate |
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Substrate Type:
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Beta-Gal Substrate |
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Target Enzyme:
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Beta-Galactosidase |
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Detection Method:
|
Fluorescent |
储存
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Store in freezer (-5 to -30°C) and protect from light. |
The DetectaGene™ Green lacZ Gene Expression Kit includes a fluorescein-based β-galactose derivative that has been chemically modified to include a mildly thiol-reactive chloromethyl group. Once inside a cell, the DetectaGene™ substrate undergoes two reactions: 1) its two galactose moieties are cleaved by intracellular β-galactosidase and 2) either simultaneously or sequentially, its chloromethyl moiety reacts with glutathione and possibly other intracellular thiols to form a membrane-impermeant, peptide-fluorescent dye adduct.
