General description
Stabilin-1 and Stabilin-2 constitute a novel family of fasciclin domain-containing hyaluronan receptor homologs that may play a role in cell-cell and cell-extracellular matrix interactions in vascular function and inflammatory processes. Stabilin-1 is a transmembrane protein, expressed in endothelial cells within the sinusoidal regions of liver, spleen, and lymph tissues as well as macrophages. Stabilin-2 is absent from the latter (Politz, 2002).
~ 275 kDa
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human Stabilin-1.
Application
Anti-Stabilin-1 Antibody, C-terminus is an antibody against Stabilin-1 for use in WB, IH(P) & IF.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Stabilin-1 in human placental tertiary villi.
Immunofluorescence Analysis: A previous lot of Anti-Stabilin-1, C-terminus was shown to positively stain using immunofluorescence techniques (Kzhyshkowska, 2008).
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
Biochem/physiol Actions
Other notable species homologies include mouse (87%) and rat (83%).
The antibody recognizes the C-terminus of Stabilin-1 within the cytoplasmic region of a cell.
Physical form
Format: Unpurified
Unpurified
Unpurified rabbit polyclonal in serum with 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt if stored in undiluted aliquots.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HUVEC lysate.
Evaluated by Western blot in HUVEC lysate.
Western blot Analysis: 1:1,000 dilution of this antibody detected Stabilin-1 on 10 µg of HUVEC lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | unpurified |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| UniProt accession no. | Q9NY15 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... STAB1(23166) |
