General description
Protein sprouty homolog 1 (UniProt O43609; also known as Spry-1) is encoded by the SPRY1 gene (Gene ID 10252) in human. Sprouty was originally identified in Drosophila as a negative regulator of fibroblast growth factor (FGF) signaling during tracheal development. There exist four mammalian homologs (SPRY1-4) that likewise mediate feedback inhibition against growth factor-/receptor tyrosine kinase- (RTK-) dependent Ras/mitogen-activated protein (MAP) kinase signaling pathway. Loss of function studies in mice show that SPRY1 and SPRY2 play critical roles during early development of multiple organ rudiments, including external genitalia, eye, kidney, inner ear, tooth, and cortical patterning in the brain. In addition, evidences indicate that SPRY1 plays an antiproliferative and tumor-suppressing role in cancers. SPRY1 downregulation is reported in various cancer types, including prostate, breast, and ovarian cancers, and SPRY1 expresion has been proposed as a marker of good prognosis in patients with renal cell carcinoma.
~35 kDa observed. 35.12 kDa calculated.
Immunogen
GST-tagged recombinant human SPRY1 N-terminal fragment.
Application
Research Category
Stem Cell Research
This mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPRY1 in 10 µg of SK-MEL-2 human skin melanoma cell lysate.
Biochem/physiol Actions
Clone 7E6.2 detects the ~35 kDa human SPRY1 by targeting an N-terminal region epitope.
Physical form
Mouse IgG1 hybridoma culture supernatant without azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected SPRY1 in 10 µg of HepG2 human hepatocellular carcinoma cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | 7E6.2, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001244967 |
| UniProt accession no. | O43609 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... SPRY1(10252) |
