General description
The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
Application
Anti-CD45.1 (mouse), APC-Cy7, clone A20 | MABF433 is an antibody against CD45.1 for use in Immunoprecipitation, Immunohistochemistry, Flow Cytometry.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 1 μg of this antibody detected CD45.1 in one million SJL splenocytes.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | APC-Cy7 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | A20, monoclonal |
| species reactivity | mouse |
| concentration | 0.2 mg/mL |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2aκ |
| UniProt accession no. | P06800 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ptprc(19264) |
