Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Study of the Class III CD34 epitope by FACS
Optimal working dilutions must be determined by the end user.
This Anti-CD34 Antibody, Class III, clone 581 is validated for use in FC for the detection of CD34 Class III.
Biochem/physiol Actions
Clone 581 reacts with the class III CD34 epitope is resistant to neuraminidase, chymopapain and glycoprotease.
The antibody reacts with a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein which is expressed on haematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. CD34 may also play a role in the adhesion of specific antigens to endothelium.
Physical form
Format: Purified
Protein G affinity chromatography. The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Preparation Note
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | 581, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001025109.1,NM_001773.2 |
| UniProt accession no. | P28906 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CD34(947) |
