General description
Surfactant Protein B (SP-B) is mostly composed of phospholipids, but also incorporates essential proteins. It is necessary for pulmonary surfactant function and the alveolar type 2 cell phenotype. SP-B deficiency has been known to cause congenital pulmonary alveolar proteinosis. SP-B is thought to protect lungs against endotoxin-induced inflammation. SP-B could be used as a clinical marker in chronic heart failure.
~16 kDa observed
Immunogen
Epitope: Saposin B-type 2 domain
GST-tagged recombinant protein corresponding to human Surfactant Protein B at the Saposin B-type 2 domain.
Application
Immunohistochemistry Analysis: 1:200 dilution from a representative lot detected Surfactant protein B in clara cells in normal rat lung tissue.
Research Category
Signaling
Research Sub Category
Lipid Signaling
This Anti-Surfactant Protein B Antibody is validated for use in WB, IH(P) for the detection of Surfactant Protein B.
Biochem/physiol Actions
This antibody recognizes Surfactant Protein B at the Saposin B-type 2 domain.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human lung tissue lysate
Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 1:5,000 dilution of this antibody detected Surfactant Protein B on 10 µg of human lung tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, mouse, human |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_942140 |
| UniProt accession no. | P07988 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SFTPB(6439) |
