General description
Eps15, also known as Epidermal growth factor receptor substrate 15, Protein AF-1p, and encoded by the gene EPS15/AF1P, is an important protein involved in cell growth regulation, cell spreading, proliferation, invagination and budding. Epidermal growth factor receptor pathway substrate 15 (Eps15) has been suggested to be involved in the endocytosis of cell surface receptors, including epidermal growth factor receptor (EGFR). Eps15 also plays a role in the assembly of clathrin-coated pits and endocytosis. Eps15 is localized in the cytoplasm and plasma membrane and in particular to clathrin coated pits upon EGFR activation. Eps15 is phosphorylated at Tyr-849 by p38alpha kinase upon stimulation with EGF during endocytic processes. Eps15 is ubiquitously expressed and a chromosomal aberration involving the Eps15 gene is associated with acute leukemia.
~125 kDa observed
Immunogen
Linear peptide corresponding to human Eps15.
Application
Anti-Eps15 Antibody is an antibody against Eps15 for use in western blotting, ICC & IHC.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Eps15 in 10 µg of A431, HeLa, HepG2, mouse brain, and PC12 cell lysate.
Western Blotting Analysis: A representative lot detected Eps15 in NIH/3T3 cell lysate (Fazioli, F., et al. (1993). Molecular and Cellular Biology. 13(9):5814-5828).
Western Blotting Analysis: A representative lot detected Eps15 in GST cell lysate (Umasankar, P. K., et al. (2012). Nature. 14(5):488-501).
Immunocytochemistry Analysis: A representative lot detected Eps15 in HeLa cells (Hinrichsen, L., et al. (2003). JBC. 278:45160-45170).
Immunohistochemistry Analysis: A representative lot detected Eps15 in glomerulus tissue. (Hasse, S., et al. (2006). Cell Biology. 126:219-229).
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Eps15 in 10 µg of HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, rat, mouse |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_001153441 |
| UniProt accession no. | P42566 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... EPS15(2060) |
