General description
Ubiquitin carboxyl-terminal hydrolase 16 (UniProt: Q9Y5T5; also known as EC:3.4.19.12, Deubiquitinating enzyme 16, Ubiquitin thioesterase 16, Ubiquitin-processing protease UBP-M, Ubiquitin-specific-processing protease 16) is encoded by the USP16 (also known as MSTP039) gene (Gene ID: 10600) in human. USP16 specifically deubiquitinates lysine 120 of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is reported to be a prerequisite for subsequent phosphorylation at serine 11 of histone H3 (H3S10ph) and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by Aurora kinase B leads to enhancement in its deubiquitinase activity, thereby maintaining transcription in resting lymphocytes. USP16 also regulates Hox gene expression via histone H2A deubiquitination. It is not shown to deubiquitinate histone H2B. USP16 can contribute to somatic stem cell defects observed in Down syndrome. USP16 is triplicated in Down syndrome and its overexpression contributes to proliferation defects in stem cells. Reduction of USP16 levels results in increased proliferation capacity of Down syndrome fibroblasts. (Ref.: Joo, HY., et al. (2007). Nature. 449(7165);1068-1072).
Immunogen
GST-tagged recombinant fragment corresponding to the first 257 amino acids from the N-terminal half of human Ubiquitin carboxyl-terminal hydrolase 16 (USP16).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected UBP16 in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected UBP16 in lysates from wild-type HEK293T cells, but not in lysate from HEK293T cells with USP16 knockout.
ELISA Analysis: A representative lot detected UBP16 in ELISA applications. (Yang, W., et. al. (2014). Nat Commun. 5:3818).
Immunohistochemistry Applications: A representative lot detected USP16 in Immunohistochemistry applications. (Yang, W., et. al. (2014). Nat Commun. 5:3818).
Flow Cytometry Analysis: A representative lot detected UBP16 in Flow Cytometry applications (Gu, Y., et. al. (2016). Proc. Natl. Acad. Sci. USA. 113(1); E51-60).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-USP16, Cat. No. ABS2211, is a rabbit polyclonal antibody that detects USP16 and is tested for use in ELISA, Flow Cytometry, Immunohistochemistry, and Western Blotting.
Biochem/physiol Actions
This rabbit polyclonal antibody detects Ubiquitin carboxyl-terminal hydrolase 16 (USP16). It targets an epitope within the N-terminal half.
Physical form
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Preparation Note
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | N/A, polyclonal,polyclonal |
| mol wt | calculated mol wt 93.57 kDa,observed mol wt ~105 kDa |
| species reactivity | human |
| packaging | antibody small pack of 100 μg |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunohistochemistry (frozen sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_001001992.1 |
| UniProt accession no. | Q9Y5T5 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... USP16(10600) |
