General description
Hox, Pbx, and Meis families of transcription factors form heteromeric complexes and bind DNA through specific homeobox domains. Meis1, 2, and 3 are members of the TALE (three amino acid loop extension) family of homeodomain containing proteins. Meis1 binds to Hox and Pbx proteins to form complexes with distinct DNA-binding specificities. Like Hox and Pbx proteins, the overexpression of Meis1 in BHX-2 myeloid leukemias implies a role for this protein in oncogenesis. Two Meis related proteins, Meis2 and Meis3 (also designated Mrg1 and Mrg2, respectively), possess largely similar sequence identity with Meis1 and express in normal tissues as well as myeloid leukemias. In the pancreas, Meis2 preferentially associates with Pbx1, and together they associate with the pancreas specific homeodomain factor, Pdx1, to repress Pdx1-induced transcriptional activation.
Mr ~45-66kDa
Immunogen
MBP-conjugated fusion protein corresponding to amino acids 60-390 of mouse Meis1
Application
Detect Meis 1/2/3 with Anti-Meis 1/2/3 Antibody, clone 9.2.7 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Biochem/physiol Actions
Meis1, Meis2, Meis3 and splice variants
Physical form
Ascites
mouse ascites IgG1 containing 0.05% sodium azide before the addition of glycerol to 30%
Preparation Note
2 years at -20°C
Analysis Note
Control
K562 nuclear extract
routinely evaluated by immunoblot on nuclear extract from K562 cells
Other Notes
Replaces: MABN1071
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 9.2.7, monoclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_170677 |
| UniProt accession no. | O14770 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MEIS2(4212) |
