General description
IGFBP2 (insulin-like growth factor binding protein 2) belongs to the IGFBP family of proteins. The protein has two HBD (heparin-binding domain-1) domains. It is a secreted protein.
Biochem/physiol Actions
During development, insulin-like growth factor binding protein-2 is expressed in a number of tissues with the highest expression level found in the central nervous system. IGFBP-2 exhibits a 2-10 fold higher affinity for IGF II than for IGF I. Mouse and human IGFBP-2 share approximately 82% homology.
Exhibits a 2-10 fold higher affinity for IGF II than for IGF I.
IGFBP2 (insulin-like growth factor binding protein 2) works along with IGF-I (insulin like growth factor I) and regulates cellular proliferation and differentiation. It mainly binds to IGFs and restricts their association to other receptors.
Physical form
Lyophilized from a 0.2 μm filtered solution of 35% acetonitrile and 0.1% TFA.
Analysis Note
The biological activity is measured by its ability to inhibit recombinant human lGF-II on MCF-7 cells.
| biological source | mouse |
| Quality Segment | 200 |
| recombinant | expressed in NSO cells |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.125-0.5 mg per mL ED50 |
| mol wt | ~35 kDa by SDS-PAGE (reducing),calculated mol wt 29.4 kDa |
| packaging | pkg of 25 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | D3YU40 |
| storage temp. | −20°C |
| Gene Information | mouse ... Igfbp2(16008) |
