General description
Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT), a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson′s disease, Tourette′s disease, schizophrenia, and addiction. DAT is also the target of several “DAT-blockers” including amphetamines and cocaine. These chemicals inhibit the action of DAT and, to a lesser extent, the other monoamine transporters, but their effects are mediated by separate mechanisms.
~ 50 – 80 kDa
Reduced form at approx 50 kDa and Non-reduced form at approx. 80 kDa
Application
This Anti-Dopamine Transporter, 100 µg Antibody is validated for use in WB for the detection of Dopamine Transporter, 100 µg.
Biochem/physiol Actions
Cat. AB2231 recognizes the N-terminus of the reduced and non reduced forms of DAT.
Physical form
Format: Purified
Analysis Note
Routinelyevaluated by western blot on rat brain lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_001044.3 |
| UniProt accession no. | Q01959 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Slc6A3(13162) rat ... Slc6A3(24898) |
