General description
C-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A
CDK2/cyclinA
Biochem/physiol Actions
Protein Target: CDK2/cyclinA
Target Sub-Family: CMGC
Packaging
Also available in 10 μg size and in bulk
Also available in 250μg size, please inquire for pricing and availability.
Physical form
The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose™ and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose
Preparation Note
6 months at -70°C
Analysis Note
routinely evaluated by phosphorylation of Histone H1
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Replaces: 14-407
Legal Information
Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| product line | Upstate® |
| specific activity | (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.) |
| mol wt | Mw 35 kDa |
| purified by | (The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose) |
| species reactivity | human |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| accession no. | (FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. ),NM_001798.2 |
| NCBI accession no. | NM_001798.2,NM_052827.1 |
| UniProt accession no. | P24941 |
