Immunogen
Chicken gizzard actin
Epitope: smooth muscle gamma & alpha actin
Application
Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P).
Western Blot: 1:1000 (approximately 43 kDa)
Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)
Optimal dilutions must be determined by the end user.
Biochem/physiol Actions
Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | ascites fluid |
| clone | CGA7, monoclonal |
| species reactivity | human, rat, chicken, rabbit, monkey |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_001613.1 |
| UniProt accession no. | P62736,P63267 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
