General description
Alpaca Anti-Mouse IgG3(Fcγ) HRP single domain Antibody is designed to specifically detect the Fc region of mouse IgG3. It is a monovalent, single domain, recombinant antibody to mouse IgG3(Fcγ fragment specific) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG3(Fcγ) HRP single domain Antibody detects the Fc region of mouse IgG3 selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Immunogen
Recombinant Alpaca single variable domain secondary nano antibody
Biochem/physiol Actions
Mouse IgG3(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c
Packaging
100 μg in Glass bottle
Physical form
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
Preparation Note
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C
Other Notes
No cross-reactivity with mouse IgG1/2a/2b, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
| biological source | alpaca |
| recombinant | expressed in E. coli |
| conjugate | horse radish peroxidase (HRP) |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | recombinant monoclonal |
| form | lyophilized solid |
| mol wt | 14 kDa |
| species reactivity | mouse, Alpaca |
| technique(s) | ELISA: 1:10000-1:50000,western blot: 1:10000-1:50000 |
| size | 0.1 mL |
| color | colorless |
| isotype | VHH |
| shipped in | wet ice |
| storage temp. | 2-8°C |
