General description
RIG1 is a cytoplasmically bound enzyme that exhibits phospholipase 1/2 activity and catalyzes the hydrolysis of various important membrane lipids including phosphatidylcholine and phosphatidylethanolamine containing lipids. RIG1 mimics PLA1/2 activities in many tissues and is widely expressed. Recent research has shown that RIG1 also functions as a cytoplasmic pattern receptor that detects viral RNA and acts as a cellular sensor for viral infection, which in turn can activate the proinflammatory response and the production of type-1 interferons against the infection.
~17 kDa observed
Immunogen
Recombinant protein corresponding to human RIG1.
Application
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected RIG1 in 1X10E6 THP-1 cells.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-RIG1 Antibody, clone 42 is validated for use in Western Blotting and Flow Cytometry for the detection of RIG1.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected RIG1 in 10 µg of THP-1 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 42, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_004576 |
| UniProt accession no. | Q9UL19 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PLAAT4(5920) |
