General description
The protein named Proteinase-activated receptor 2 (PAR2/PAR-2), or Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like-1 and encoded by the human gene F2RL1/GPR11/PAR2 is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Also as part of the innate and adaptive immunity response, PAR2 acts as sensor for proteolytic enzymes generated during infection and works to promote inflammation and the inflammatory response. PAR2 is also involved in vascular and airway tone and in blood pressure regulation and many other G protein coupled receptor response pathways. PAR2 is a multi-pass membrane protein that is widely expressed in most tissues except brain or skeletal muscle.
~50 kDa observed. The calculated MW of this protein is 44 kDa However, it may be observed at higher molecular weights due to glycosylation.
Immunogen
Epitope: N-terminus
Linear peptide corresponding to the N-terminus of human Par-2.
Application
Anti-Par-2 Antibody, clone SAM11 is an antibody against Par-2 for use in western blotting, IHC & flow cytometry.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Par-2 in human small intestine, coronary artery and aorta sections, and in human artery and vein tissues (Molino, M., et al. (1998). Arterioschler Throm Vasc Biol. 18(5):825-832.).
Flow Cytometry Analysis: Arepresentative lot from an independent laboratory detected Par-2 in Par-2 transfected COS-1 cells (Molino, M., et al. (1997). J Biol Chem. 272(17):11133-11141.).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Par-2 in 10 µg of NIH/3T3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | SAM11, monoclonal |
| species reactivity | mouse, human |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_005233 |
| UniProt accession no. | P55085 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... F2RL1(2150) |
