General description
26 kDa
The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.
Immunogen
Epitope: Ser70
KLH-conjugated linear peptide corresponding to human phosphorylated Bcl-2 at Ser70.
Application
Detect phospho-Bcl-2 (Ser70) using this Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in WB, IP, FC.
Immunoprecipitation Analysis: 1ug from a previous lot immunoprecipitated phosphorylated Bcl-2 from 100 ug of jurkat lysate treated with paciltaxel. Arrow indicates phospho-Bcl-2 (~26kD).
Flow Cytometry Analysis: 0.1ug/ml from a previous lot was used to detect Bcl-2 in Jurkat cell lysate.
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
Biochem/physiol Actions
This antibody recognizes phosphorylated Bcl-2 at Ser70.
Physical form
Protein A purified
Purified rabbit monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% Glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Paclitaxel treated and untreated Jurkat cell lysate
Evaluated by Western Blot in paclitaxel treated and untreated Jurkat cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Bcl-2 on 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 69-10C-2-10C-18, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_000624.2 |
| UniProt accession no. | P10415 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pSer70) |
| Gene Information | human ... BCL2(596) |
