General description
The acute lymphoblastic leukaemia (ALL)-1 (also known as MLL, HRX, HTRX, and TRX1) gene on human chromosome 11q23 is the site of many locally clustered chromosomal alterations associated with several types of acute leukaemias, including deletions, partial duplications and translocations. Structurally variant proteins derived from the altered gene presumably cause the malignant transformation of early haemopoietic progenitor cells.
300 kDa
Immunogen
MBP fusion protein corresponding to residues 161-356 of human mixed lineage leukemia, MLL
Application
Detect MLL/HRX with Anti-MLL/HRX Antibody, N-term., clone N4.4 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
Biochem/physiol Actions
Recognizes N-terminal proteolytic fragment of MLL (MLLN).
Physical form
Format: Purified
Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
Analysis Note
routinely evaluated by immunoblot on nuclear extract from K562 cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | N4.4, monoclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_005933 |
| UniProt accession no. | Q03164 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... KMT2A(4297) |
