General description
Calcyclin, also known as S100A6, is a calcium binding protein that is highly expressed in fibroblasts and epithelial cells. Inhibition of calcyclin by p53 is part of the mechanism to control cell proliferation. Calcyclin is associated with cancer and metastasis and plays a role in cell cycle regulation.
~10 kDa observed
Immunogen
Full length recombinant protein corresponding to human Calcyclin.
Material obtained from the National Cancer Institute’s Clinical Proteomic Technologies for Cancer (CPTC) program.
Application
Anti-Calcyclin Antibody, clone CPTC-Calcyclin-2 detects level of Calcyclin & has been published & validated for use in WB, ELISA, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Tara Hitke, Developmental Studies Hybridoma Bank, University of Iowa.)
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human lung tissue lysate
Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Calcyclin on 10 µg of human lung tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | CPTC-Calcyclin-2, monoclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_055439 |
| UniProt accession no. | P06703 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... S100A6(6277) |
