General description
Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.
Immunogen
Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.
Application
Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.
For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.
Research Category
Apoptosis & Cancer
Metabolism
Research Sub Category
Apoptosis - Additional
Enzymes & Biochemistry
Biochem/physiol Actions
Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.
The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.
Physical form
Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
ImmunoAffinity Purified
Preparation Note
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
Analysis Note
Control
Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).
Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.
Other Notes
Replaces: 04-575; 04-576
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, bovine, rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_001618.3 |
| UniProt accession no. | P09874 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | bovine ... Parp1(286764) human ... PARP1(142) mouse ... Parp1(11545) rat ... Parp1(25591) |
