Immunogen
Recombinant PrP amino acids 23-237.
Application
Anti-Prion Protein Antibody is an antibody against Prion Protein for use in ELISA, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:50,000-1:200,000
Immunohistochemistry: 1:100-1:500. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
a. formic acid for 10 minutes at room temperature
b. hydrolytic autoclaving
c. heat induced epitope retrieval
ELISA: 1:20,000-1:50,000
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with prion protein (PrP) from bovine, sheep, mule deer, elk and mouse. The monoclonal recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.
Preparation Note
Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt.
Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | monoclonal |
| species reactivity | sheep, mouse, mule deer, bovine, elk |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_000311.3,NM_001080121.1,NM_001080122.1,NM_001080123.1,NM_183079.2 |
| UniProt accession no. | P04156 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PRNP(5621) |
