General description
Immunogen: GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH.
Specificity: Recognizes GST-fusion proteins.
Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
Immunogen
GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH. Clone DG122-2A7.
Application
Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate detects level of GST Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Biochem/physiol Actions
Recognizes GST-fusion proteins.
Physical form
Protein G Purified
Purified mouse monoclonal IgG in buffer containing (HRP) in 70% storage buffer PBS with 0.05% preservative and 30% glycerol. Liquid at -20°C.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Cells expressing the GST-tag.
Routinely evaluated by western blot Analysis on recombinant GST protein.
Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | peroxidase conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | DG122-2A7, monoclonal |
| species reactivity | Schistosoma japonicum |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_001070 |
| UniProt accession no. | P43403 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
