General description
Retinoid-related orphan receptor-gamma t (RORγT) is one of two RORγ isoforms belonging to the nuclear hormone receptor family and contains a single nuclear receptor DNA-binding domain. RORγT is a negative regulator of TNFDF6/FASL expression and T-cell production of IL2. The upregulation of RORγT is a requirement for TH17 cell differentiation. Inhibition of RORγT is induced by TGF-β’s interaction with Foxp3. Expression is observed mainly in immature thymocytes, and, during fetal development, is found exclusively in lymphoid-inducer (LTi) cells.
~52 kDa observed
Immunogen
Epitope: Unknown
Histidine-tagged recombinant protein corresponding to mouse Retinoid-Related Orphan Receptor γ T (RORγT).
Application
Detect Retinoid-Related Orphan Receptor γ T using this Anti-Retinoid-Related Orphan Receptor γ T Antibody, clone RORg2 validated for use in WB, FC & IF.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Physical form
Format: Purified
Protein G Purified
Purified hamster monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
EL4 cell lysate
Evaluated by Western Blot in EL4 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Retinoid-Related Orphan Receptor γ T (RORγT) in 10 µg of EL4 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | hamster (Armenian) |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | RORg2, monoclonal |
| species reactivity | mouse |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_035411 |
| UniProt accession no. | P51450 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Rorc(19885) |
